elisa kit elisa kits logo
search elisa kits
elisa kit contact phone ico

Tel:+86-510-82732223

Email: info@dldevelop.com.cn

Home > ELISA kit

Human Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit

Human Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit ANGPTL2 DL-ANGPTL2-Hu HARP ARP2 Angiopoietin-Related Protein 2
0
Human Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit ANGPTL2 DL-ANGPTL2-Hu HARP ARP2 Angiopoietin-Related Protein 2 code image

two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

Traditional ELISA Kit Ready-to-Use ELISA KIT
Product name: Human Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit
Method: Sandwich
Synonyms:

HARP; ARP2; Angiopoietin-Related Protein 2

Detection range: 0.312-20ng/mL
Reactivity: ANGPTL2
Size: 96T/48T
Quality guarantee period: for 12 months
Catalog number: DL-ANGPTL2-Hu (traditional) (ready-to-use)
Assay length 1-4.5Hours 1-3.5Hours
Advantages:
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at 4℃
  • Faster reaction compare to other brands
  • 12 months shelf life
Instruction Manual DL-ANGPTL2-Hu.pdf DL-ANGPTL2-Hu.pdf
Human Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit elisa kit elisa kits
1.Overview:

Other names:HARP; ARP2; Angiopoietin-Related Protein 2

Function: Induces sprouting in endothelial cells through an autocrine and paracrine action.

Sequence

 50 MRPLCVTCWW LGLLAAMGAV AGQEDGFEGT EEGSPREFIY LNRYKRAGES       
100 QDKCTYTFIV PQQRVTGAIC VNSKEPEVLL ENRVHKQELE LLNNELLKQK       
150 RQIETLQQLV EVDGGIVSEV KLLRKESRNM NSRVTQLYMQ LLHEIIRKRD     
200 NALELSQLEN RILNQTADML QLASKYKDLE HKYQHLATLA HNQSEIIAQL      
250 EEHCQRVPSA RPVPQPPPAA PPRVYQPPTY NRIINQISTN EIQSDQNLKV       
300 LPPPLPTMPT LTSLPSSTDK PSGPWRDCLQ ALEDGHDTSS IYLVKPENTN      
350 RLMQVWCDQR HDPGGWTVIQ RRLDGSVNFF RNWETYKQGF GNIDGEYWLG       
400 LENIYWLTNQ GNYKLLVTME DWSGRKVFAE YASFRLEPES EYYKLRLGRY       
450 HGNAGDSFTW HNGKQFTTLD RDHDVYTGNC AHYQKGGWWY NACAHSNLNG       
490 VWYRGGHYRS RYQDGVYWAE FRGGSYSLKK VVMMIRPNPN TFH                                  
2.Features
INTENDED USE
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANGPTL2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

DETECTION RANGE
0.312-20ng/mL. The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL.
 
 SENSITIVITY
The minimum detectable dose of ANGPTL2 is typically less than 0.134ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
 
 
 SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of ANGPTL2.
No significant cross-reactivity or interference between ANGPTL2 and analogues was observed.
You can reference link of the kit as following
http://www.dldevelop.com/Research-reagent/dl-angptl2-hu.html
http://www.dldevelop.com/Research-reagent/dl-angptl2-mu.html
http://www.dldevelop.com/Research-reagent/dl-angptl2-ra.html 
Introduction
ItemStandardTest
Description

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANGPTL2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Conform
IdentificationColorimetricPositive
Composition Traditional ELISA Kit Ready-to-Use ELISA KITConform
Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
Plate sealer for 96 wells 2Plate sealer for 96 wells 2
Standard 2 Standard 2
Diluents buffer 1×45mLStandard Diluent 1×20mL
Detection Reagent A 1×120μLDetection Solution A 1×12mL
Detection Reagent B 1×120μLDetection Solution B 1×12mL
TMB Substrate 1×9mLTMB Substrate 1×9mL
Stop Solution 1×6mLStop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mL
Instruction manual 1 Instruction manual 1

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Recovery

Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-93 86
EDTA plasma(n=5) 80-97 88
heparin plasma(n=5) 90-101 95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma(n=5) 88-101% 86-95% 90-102% 80-93%
heparin plasma(n=5) 80-91% 82-90% 95-104% 79-95%

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Order or get a Quote

We will reply you within 24 hours!

Copyright @ Wuxi Donglin Sci & Tech Development Co.,Ltd. All Rights Reserved Elisa Kit|Elisa Kits Language:Chinese

苏ICP备06050612号-1 苏公网安备 32020302000048号