Estradiol (E2)
Recently, a study in the journal BMC Cancer examined whether miRNA is involved in hormonal regulation of estrogen receptor gene expression. Western blot and quantitative PCR were used to determine the expression of estrogen responsive gene and miRNA, respectively. Luciferase reporter gene detection and transfection of miRNA or inhibition of miRNA to determine miRNA regulated target gene expression. Proliferation activity was detected by routine MTS. It was found that E2 can significantly induce the expression of bcl-2, cyclin D1 and Survivin by inhibiting a series of miRNA (mir-16, mir-143 and mir-203) levels in McF-7 cells. The miRNA transfection technique and luciferase assay confirmed that bcl-2 was regulated by mir-16 and mir-143, while cyclinD1 was regulated by mir-16. Importantly, mir-16, mir-143 and mir-203 all target the regulatory protein survivin.
The regulatory effects of E2 can be eliminated by transfection of siRNA with anti-estrogen agents, raloxifene or estrogen-receptor alpha, suggesting that regulation depends on estrogen-receptor alpha. To investigate the functional significance of these miRNAs in estrogen receptor cells, the McF-7 cells were transfected with miRNA. These miRNA overexpression inhibited E2 induced cell proliferation. Further studies showed that mir-16, mir-143 and mir-203 were highly expressed in triple positive breast cancer tissues, suggesting that these mirnas are a potential tumor suppressor in ER positive breast cancer.In conclusion, E2 was down-regulated by regulating miRNA induced bcl-2, cyclin D1 and survivin.
At present, DLDEVELOP co. LTD has developed corresponding E2 Elisa products. To get more information, you could contact our professional staff directly or directly to our website:
http://www.dldevelop.com/Research-reagent/dl-e2-ge.html
http://www.dldevelop.com/uploadfile/data/DL-E2-Ge.pdf
The regulatory effects of E2 can be eliminated by transfection of siRNA with anti-estrogen agents, raloxifene or estrogen-receptor alpha, suggesting that regulation depends on estrogen-receptor alpha. To investigate the functional significance of these miRNAs in estrogen receptor cells, the McF-7 cells were transfected with miRNA. These miRNA overexpression inhibited E2 induced cell proliferation. Further studies showed that mir-16, mir-143 and mir-203 were highly expressed in triple positive breast cancer tissues, suggesting that these mirnas are a potential tumor suppressor in ER positive breast cancer.In conclusion, E2 was down-regulated by regulating miRNA induced bcl-2, cyclin D1 and survivin.
At present, DLDEVELOP co. LTD has developed corresponding E2 Elisa products. To get more information, you could contact our professional staff directly or directly to our website:
http://www.dldevelop.com/Research-reagent/dl-e2-ge.html
http://www.dldevelop.com/uploadfile/data/DL-E2-Ge.pdf
