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Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit

Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit ABCC3 DL-ABCC3-Hu ABC31 MLP2 MOATD MRP3 cMOAT2 Canalicular Multispecific Organic Anion Transporter 2 Multi-specific organic anion transporter D Multidrug resistance-associated protein 3
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Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit ABCC3 DL-ABCC3-Hu ABC31 MLP2 MOATD MRP3 cMOAT2 Canalicular Multispecific Organic Anion Transporter 2 Multi-specific organic anion transporter D Multidrug resistance-associated protein 3 code image

two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

Traditional ELISA Kit Ready-to-Use ELISA KIT
Product name: Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit
Method: Sandwich
Synonyms:

ABC31; MLP2; MOATD; MRP3; cMOAT2; Canalicular Multispecific Organic Anion Transporter 2; Multi-specific organic anion transporter D; Multidrug resistance-associated protein 3

Detection range: 0.312-20ng/mL
Target Protein: ABCC3
Size: 96T/48T
Quality guarantee period: for 12 months, 16 months
Catalog number: DL-ABCC3-Hu (traditional) (ready-to-use)
Assay length 1-4.5Hours 1-3.5Hours
Advantages:
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at -20℃
  • Faster reaction compare to other brands
  • 16 months shelf life
Instruction Manual DL-ABCC3-Hu.pdf DL-ABCC3-Hu.pdf
Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit elisa kit elisa kits
1. Overview:

Other names:ABC31; MLP2; MOATD; MRP3; cMOAT2; Canalicular Multispecific Organic Anion Transporter 2; Multi-specific organic anion transporter D; Multidrug resistance-associated protein 3

Function: May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.

Sequence

MDALCGSGEL  GSKFWDSNLS  VHTENPDLTP  CFQNSLLAWV  PCIYLWVALP  

CYLLYLRHHC  RGYIILSHLS  KLKMVLGVLL  WCVSWADLFY  SFHGLVHGRA  

PAPVFFVTPL  VVGVTMLLAT  LLIQYERLQG  VQSSGVLIIF  WFLCVVCAIV  

PFRSKILLAK  AEGEISDPFR  FTTFYIHFAL  VLSALILACF  REKPPFFSAK  

NVDPNPYPET  SAGFLSRLFF  WWFTKMAIYG  YRHPLEEKDL  WSLKEEDRSQ  

MVVQQLLEAW  RKQEKQTARH  KASAAPGKNA  SGEDEVLLGA  RPRPRKPSFL  

KALLATFGSS  FLISACFKLI  QDLLSFINPQ  LLSILIRFIS  NPMAPSWWGF  

LVAGLMFLCS  MMQSLILQHY  YHYIFVTGVK  FRTGIMGVIY  RKALVITNSV  

KRASTVGEIV  NLMSVDAQRF  MDLAPFLNLL  WSAPLQIILA  IYFLWQNLGP  

SVLAGVAFMV  LLIPLNGAVA  VKMRAFQVKQ  MKLKDSRIKL  MSEILNGIKV  

LKLYAWEPSF  LKQVEGIRQG  ELQLLRTAAY  LHTTTTFTWM  CSPFLVTLIT 

LWVYVYVDPN  NVLDAEKAFV  SVSLFNILRL  PLNMLPQLIS  NLTQASVSLK  

RIQQFLSQEE  LDPQSVERKT  ISPGYAITIH  SGTFTWAQDL  PPTLHSLDIQ  

VPKGALVAVV  GPVGCGKSSL  VSALLGEMEK  LEGKVHMKGS  VAYVPQQAWI  

QNCTLQENVL  FGKALNPKRY  QQTLEACALL  ADLEMLPGGD  QTEIGEKGIN  

LSGGQRQRVS  LARAVYSDAD  IFLLDDPLSA  VDSHVAKHIF  DHVIGPEGVL  

AGKTRVLVTH  GISFLPQTDF  IIVLADGQVS  EMGPYPALLQ  RNGSFANFLC  

NYAPDEDQGH  LEDSWTALEG  AEDKEALLIE  DTLSNHTDLT  DNDPVTYVVQ  

KQFMRQLSAL  SSDGEGQGRP  VPRRHLGPSE  KVQVTEAKAD  GALTQEEKAA  

IGTVELSVFW  DYAKAVGLCT  TLAICLLYVG  QSAAAIGANV  WLSAWTNDAM  

ADSRQNNTSL  RLGVYAALGI  LQGFLVMLAA  MAMAAGGIQA  ARVLHQALLH  

NKIRSPQSFF  DTTPSGRILN  CFSKDIYVVD  EVLAPVILML  LNSFFNAIST  

LVVIMASTPL  FTVVILPLAV  LYTLVQRFYA  ATSRQLKRLE  SVSRSPIYSH  

FSETVTGASV  IRAYNRSRDF  EIISDTKVDA  NQRSCYPYII  SNRWLSIGVE  

FVGNCVVLFA  ALFAVIGRSS  LNPGLVGLSV  SYSLQVTFAL  NWMIRMMSDL  

ESNIVAVERV  KEYSKTETEA  PWVVEGSRPP  EGWPPRGEVE  FRNYSVRYRP 

GLDLVLRDLS  LHVHGGEKVG  IVGRTGAGKS  SMTLCLFRIL  EAAKGEIRID 

GLNVADIGLH  DLRSQLTIIP  QDPILFSGTL  RMNLDPFGSY  SEEDIWWALE 

LSHLHTFVSS  QPAGLDFQCS  EGGENLSVGQ  RQLVCLARAL  LRKSRILVLD 

EATAAIDLET  DNLIQATIRT  QFDTCTVLTI  AHRLNTIMDY  TRVLVLDKGV  

VAEFDSPANL  IAARGIFYGM  ARDAGLA

2. Features
 
INTENDED USE
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ABCC3 in human tissue homogenates, cell lysates and other biological fluids.

DETECTION RANGE
0.312-20ng/mL. The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL.
 
SENSITIVITY
The minimum detectable dose of ABCC3 is typically less than 0.105ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
 
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of ABCC3.
No significant cross-reactivity or interference between ABCC3 and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between ABCC3 and all analogues, therefore, cross reactivity may still exist.

IMPORTANT NOTES
1. Limited by the current conditions and scientific technology, it is impossible to conduct comprehensive identification and analysis tests on the raw materials provided by suppliers. As a result, it is possible there are some qualitative and/or technical risks.
2. The final experimental results will be closely related to the validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available to obtain accurate results.
3. Kits from different batches may be a little different in detection range, sensitivity and color developing time. Please perform the experiment exactly according to the instruction manual included in your kit. Electronic ones on our website are for reference only.
4. Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
5. Protect all reagents from strong light during storage and incubation. All bottle caps of reagents should be closed tightly to prevent evaporation of liquids and contamination by microorganisms.
6. There may be a foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
7. Incorrect procedures during reagent preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
8. Even the same experimenter may get different results from two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before the general assay for each batch is recommended.
9. Each kit has undergone several rigorous quality control tests. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipment. Intra-assay variance among kits from different batches could arise from the above factors as well.
10. Kits from different manufacturers with the same item might produce different results, since we have not compared our products with other manufacturers.
11. The standard of the kit and immunogen used for antibody preparation are commonly recombinant proteins. Different expressed sequence, expression systems, purification methods might be used in recombinant protein preparation. Besides, there might exist differences on the screening technique of antibody and antibody pairs in our kit. Thus we can not guarantee the kit could detect recombinant protein from other companies. So, it is not recommended to use the kit for the detection of recombinant protein.
12. Validity period: 12 months.
13. The instruction manual also works with the 48T kit, but all reagents in the 48T kit are reduced by half.
 
PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.

You can reference link of the kit as following
https://www.dldevelop.com/uploadfile/data/DL-ABCC3-Hu.pdf
Introduction
ItemStandardTest
Description

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ABCC3 in human tissue homogenates, cell lysates or other biological fluids.

Conform
IdentificationColorimetricPositive
Composition Traditional ELISA Kit Ready-to-Use ELISA KITConform
Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
Plate sealer for 96 wells 2Plate sealer for 96 wells 2
Standard 2 Standard 2
Diluents buffer 1×45mLStandard Diluent 1×20mL
Detection Reagent A 1×120μLDetection Solution A 1×12mL
Detection Reagent B 1×120μLDetection Solution B 1×12mL
TMB Substrate 1×9mLTMB Substrate 1×9mL
Stop Solution 1×6mLStop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mL
Instruction manual 1 Instruction manual 1

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Recovery

Matrices listed below were spiked with certain level of recombinant ABCC3 and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-93 86
EDTA plasma(n=5) 80-97 88
heparin plasma(n=5) 90-101 95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma(n=5) 88-101% 86-95% 90-102% 80-93%
heparin plasma(n=5) 80-91% 82-90% 95-104% 79-95%

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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