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Biotinylated antibody ( Detection A) Preparation Process

  1. Dissolve the antibody which will be Biotinylated in 0.1 M CBS, PH 9.6, at a concentration of 1mg/ml.
  2. Dissolve the NHS-biotin in DMSO or sulfo-NHS-biotin in water at a concentration of 1mg/ml.
  3. Mix the antibody solution with the NHS-biotin solution at a mass ratio of 8:1 in a bottle.
  4. React for 2h-4h at room temperature.
  5. Purify the biotinylated antibody by dialysis against PBS
  6. It shoud be stored for a long time at  -20℃ after adding 40-50% glycerol.

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