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Ready-To-Use ELISA kit Validity Stability Test

Product Name: Mouse Cystatin 3 (CST3) ready-to-use ELISA Kit 
 
1. OD Value
OD Values contrast data was as below(Under right storage conditions):
7 months period of validity OD Values: 2.654, 1.780, 1.203, 0.861, 0.675, 0.492, 0.202, 0.076
13 months period of validity OD Values:2.531, 1.766, 1.273, 0.904, 0.734, 0.600, 0.488,0.077
17 months period of validity OD Values:2.451, 1.669, 1.221,  0.879, 0.656, 0.409, 0.289, 0.068
 
2. Recovery
Matrices listed below were spiked with certain level of recombinant mouse CST3 and the recovery rates were calculated by comparing the measured value to the expected amount of CST3 in samples.
 
7 months 13 months and after 17 months period of validity ELISA Kit Recovery results
Martrix Conditions Recovery range(%) Average(%)
Serum(n=6) 7 months 91-103 97
13 months 80-100 90
17 months 81-101 91
EDTA plasma(n=6) 7 months 86-106 96
13 months 78-98 88
17 months 79-102 91
 
3. Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of mouse CST3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
 
7 months 13 months and 17 months period of validity ELISA Kit Linearity results
Sample Conditions 1:2 1:4 1:8
Serum(n=6) 7 months 89-99% 81-105% 90-102%
13 months 87-95% 83-98% 86-103%
17 months 85-97% 79-99% 82-101%
EDTA plasma(n=6) 7 months 93-104% 81-102% 92-102%
13 months 89-94% 78-109% 90-96%
17 months 89-95% 76-97% 89-93%
 
4. Precision
CV(%) = SD/meanX100
Intra-assay Precision (Precision within one batch): 3 samples with low, middle and high level mouse CST3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between batches): 3 samples with low, middle and high level mouse 

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