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FAQ
Rabbit anti-SMYD3 Antibody
| Product name: | Rabbit anti-SMYD3 Antibody | |
| Catalog: | DL98173A |
|
| Synonyms : | ZMYND1; ZNFN3A1; Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1 | |
| Reactivity: | H, M | |
| Applications: | WB, IF/IC | |
| Size: | 50uL/100uL | |
| Host: | Rabbit | |
| Clonality: | Polyclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | Recombinant full length protein of human SMYD3 | |
| WB description: | Western blot analysis of SMYD3 expression in Jurkat (A), MCF7 (B), Hela (C), mouse heart (D) whole cell lysates. | |
| IF/ICC description: | Immunofluorescent analysis of SMYD3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
|
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) | |
| Gene ID(Human): | 64754 | |
| Gene ID (Mouse)): | 69726 | |
| SwissProt (Human): | Q9H7B4 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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