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FAQ
Rabbit anti-CD177 Antibody
| Product name: | Rabbit anti-CD177 Antibody | |
| Catalog: | DL98055A |
|
| Synonyms : | NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177 | |
| Reactivity: | H | |
| Applications: | WB, IF/IC | |
| Size: | 50uL/100uL | |
| Host: | Rabbit | |
| Clonality: | Polyclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | Recombinant full length protein of human CD177 | |
| WB description: | Western blot analysis of CD177 expression in BT474 (A) whole cell lysates. | |
| IF/ICC description: | Immunofluorescent analysis of CD177 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
|
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) | |
| Gene ID(Human): | 57126 | |
| SwissProt (Human): | Q8N6Q3 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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