Rabbit anti-POLR2F Antibody

Product name:	Rabbit anti-POLR2F Antibody
Catalog:	DL97623A
Synonyms :	POLRF; DNA-directed RNA polymerases I, II, and III subunit RPABC2; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; RPABC14.4; RPB14.4; RPB6 homolog; RPC15
Reactivity:	H, M, R
Applications:	WB, IF/IC
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	Recombinant full length protein of human POLR2F
WB description:	Western blot analysis of POLR2F expression in mouse liver (A) whole cell lysates.
IF/ICC description:	Immunofluorescent analysis of POLR2F staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Gene ID(Human):	5435
Gene ID (Mouse)):	69833
Gene ID(Rat):	83503
SwissProt (Human):	P61218
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL97623A