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Home > Polyclonal & monoclonal

Rabbit anti-Cytochrome P450 51A1 Antibody

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Product name: Rabbit anti-Cytochrome P450 51A1 Antibody
Catalog:

DL96788A

Synonyms : CYP51; Lanosterol 14-alpha demethylase; LDM; CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase
Reactivity: H, M, R
Applications: WB, IHC, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: Recombinant full length protein of human Cytochrome P450 51A1
WB description: Western blot analysis of Cytochrome P450 51A1 expression in mouse kidney (A), rat liver (B) whole cell lysates.
IHC description: Immunohistochemical analysis of Cytochrome P450 51A1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of Cytochrome P450 51A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/20 - 1/100)
Gene ID(Human): 1595
Gene ID (Mouse)): 13121
Gene ID(Rat): 25427
SwissProt (Human): Q16850
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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