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FAQ
Rabbit anti-H6PD Antibody
| Product name: | Rabbit anti-H6PD Antibody | |
| Catalog: | DL96243A |
|
| Synonyms : | GDH; GDH/6PGL endoplasmic bifunctional protein | |
| Reactivity: | H | |
| Applications: | WB, IHC, IF/IC | |
| Size: | 50uL/100uL | |
| Host: | Rabbit | |
| Clonality: | Polyclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | Recombinant full length protein of human H6PD | |
| WB description: | Western blot analysis of H6PD expression in mouse liver (A), mouse kidney (B) whole cell lysates. | |
| IHC description: | Immunohistochemical analysis of H6PD staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. | |
| IF/ICC description: | Immunofluorescent analysis of H6PD staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
|
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100) | |
| Gene ID(Human): | 9563 | |
| SwissProt (Human): | O95479 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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