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FAQ
Rabbit anti-PPP2R2A Antibody
| Product name: | Rabbit anti-PPP2R2A Antibody | |
| Catalog: | DL95991A |
|
| Synonyms : | Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha | |
| Reactivity: | H, M, R | |
| Applications: | WB, IHC, IF/IC | |
| Size: | 50uL/100uL | |
| Host: | Rabbit | |
| Clonality: | Polyclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | Recombinant full length protein of human PPP2R2A | |
| WB description: | Western blot analysis of PPP2R2A expression in Hela (A), Jurkat (B), mouse brain (C) whole cell lysates. | |
| IHC description: | Immunohistochemical analysis of PPP2R2A staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. | |
| IF/ICC description: | Immunofluorescent analysis of PPP2R2A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
|
| Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) | |
| Gene ID(Human): | 5520 | |
| Gene ID (Mouse)): | 71978 | |
| Gene ID(Rat): | 117104 | |
| SwissProt (Human): | P63151 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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