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Home > Polyclonal & monoclonal

Rabbit anti-DHFR Antibody

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Product name: Rabbit anti-DHFR Antibody
Catalog:

DL95924A

Synonyms : Dihydrofolate reductase
Reactivity: H, M, R
Applications: WB, IHC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: Recombinant full length protein of human DHFR
WB description: Western blot analysis of DHFR expression in Hela (A), HL60 (B), THP1 (C), mouse liver (D) whole cell lysates.
IHC description: Immunohistochemical analysis of DHFR staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene ID(Human): 1719
Gene ID (Mouse)): 13361
Gene ID(Rat): 24312
SwissProt (Human): P00374
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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