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Home > Polyclonal & monoclonal

Rabbit anti-APOBEC3G Antibody

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Product name: Rabbit anti-APOBEC3G Antibody
Catalog:

DL95914A

Synonyms : DNA dC->dU-editing enzyme APOBEC-3G; APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM-15; CEM15; Deoxycytidine deaminase; A3G
Reactivity: H, R
Applications: WB, IHC, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: Recombinant full length protein of human APOBEC3G
WB description: Western blot analysis of APOBEC3G expression in Jurkat (A) whole cell lysates.
IHC description: Immunohistochemical analysis of APOBEC3G staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:

Immunofluorescent analysis of APOBEC3G staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Gene ID(Human): 60489
SwissProt (Human): Q9HC16
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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