Rabbit anti-Alpha-enolase Antibody

Product name:	Rabbit anti-Alpha-enolase Antibody
Catalog:	DL95881A
Synonyms :	ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
Reactivity:	H, M, R
Applications:	WB, IF/IC
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	Recombinant full length protein of human Alpha-enolase
WB description:	Western blot analysis of Alpha-enolase expression in PC3 (A), Ramos (B), Hela (C), THP1 (D) whole cell lysates.
IF/ICC description:	Immunofluorescent analysis of Alpha-enolase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)
Gene ID(Human):	2023
Gene ID (Mouse)):	13806; 433182
Gene ID(Rat):	24333
SwissProt (Human):	P06733
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL95881A