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FAQ
Mouse anti-Histone H3 Antibody
| Product name: | Mouse anti-Histone H3 Antibody | |
| Catalog: | DL95527A |
|
| Synonyms : | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3 | |
| Reactivity: | H, M, R, Yeast | |
| Applications: | WB, IF/IC, IP | |
| Size: | 50uL/100uL | |
| Host: | Mouse | |
| Clonality: | Monoclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | Recombinant protein corresponding to human Histone H3. | |
| WB description: | Western blot analysis of Histone H3 expression in Hela (A), Raw264.7 (B), mouse brain (C), rat brain (D) whole cell lysates. | |
| IF/ICC description: | Immunofluorescent analysis of Histone H3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
|
| IP description: | Immunoprecipitation of Histone H3 from 0.5mg Hela whole cell extract lysate, using anti-Histone H3 Antibody. | |
| Purification_Method: | ||
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/2000 - 1/5000), IF/IC (1/100 - 1/500), IP (1/50 - 1/200) | |
| Gene ID(Human): | 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968 | |
| Gene ID (Mouse)): | 319152; 15077; 15078 | |
| Gene ID(Rat): | 291159; 100361558 | |
| SwissProt (Human): | P68431; Q71DI3; P84243 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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