Mouse anti-Cytokeratin 18 Antibody
Product name: | Mouse anti-Cytokeratin 18 Antibody | |
Catalog: | DL95486A |
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Synonyms : | CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18 | |
Reactivity: | H, M, R | |
Applications: | WB, IF/IC | |
Size: | 50uL/100uL | |
Host: | Mouse | |
Clonality: | Monoclonal | |
Concentration: | 1mg/mL | |
Immunogen: | Recombinant protein corresponding to human Cytokeratin 18. | |
WB description: | Western blot analysis of Cytokeratin 18 expression in HepG2 (A), Hela (B), mouse liver (C), mouse skeletal muscle (D), C2C12 (E), rat heart (F) whole cell lysates. | |
IF/ICC description: | Immunofluorescent analysis of Cytokeratin 18 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
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Purification_Method: | ||
Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
Dilution: | WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200) | |
Gene ID(Human): | 3875 | |
Gene ID (Mouse)): | 16668 | |
Gene ID(Rat): | 294853 | |
SwissProt (Human): | P05783 | |
Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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