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FAQ
Mouse anti-Caspase 9 Antibody
| Product name: | Mouse anti-Caspase 9 Antibody | |
| Catalog: | DL95472A |
|
| Synonyms : | MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 | |
| Reactivity: | H, M, R | |
| Applications: | WB, IF/IC, IP | |
| Size: | 50uL/100uL | |
| Host: | Mouse | |
| Clonality: | Monoclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary. | |
| WB description: | Western blot analysis of Caspase 9 expression in Hela (A), Jurkat (B), HepG2 (C), mouse heart (D), rat heart (E) whole cell lysates. | |
| IF/ICC description: | Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
|
| IP description: | Immunoprecipitation of Caspase 9 from 0.5mg Hela whole cell extract lysate, using anti-Caspase 9 Antibody. | |
| Purification_Method: | ||
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/1000 - 1/3000), IP (1/50 - 1/200) | |
| Gene ID(Human): | 842 | |
| SwissProt (Human): | P55211 | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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