Mouse anti-Alpha-actin-1 Antibody

Product name:	Mouse anti-Alpha-actin-1 Antibody
Catalog:	DL95441A
Synonyms :	ACTA; Actin alpha skeletal muscle; Alpha-actin-1
Reactivity:	H, M, R
Applications:	WB, IHC, IP
Size:	50uL/100uL
Host:	Mouse
Clonality:	Monoclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-actin-1. The exact sequence is proprietary.
WB description:	Western blot analysis of Alpha-actin-1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
IHC description:	Immunohistochemical analysis of Alpha-actin-1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IP description:	Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate, using anti-Alpha-actin-1 Antibody.
Purification_Method:
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/5000 - 1/10000), IH (1/200 - 1/500), IP (1/100 - 1/200)
Gene ID(Human):	58
Gene ID (Mouse)):	11459
Gene ID(Rat):	29437
SwissProt (Human):	P68133
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL95441A