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FAQ
Mouse anti-FLAG-tag Antibody
| Product name: | Mouse anti-FLAG-tag Antibody | |
| Catalog: | DL95390A |
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| Synonyms : | ||
| Reactivity: | ||
| Applications: | WB, IF/IC, IP | |
| Size: | 50uL/100uL | |
| Host: | Mouse | |
| Clonality: | Monoclonal | |
| Concentration: | 1mg/mL | |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag. The exact sequence is proprietary. | |
| WB description: | Western blot analysis of over-expressed HA-tagged protein in 293T cell lysate. | |
| IF/ICC description: | Immunofluorescent analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
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| IP description: | Immunoprecipitation of HA-tagged protein from HEK293T cells transfected with vector overexpressing HA tag, using anti-HA-tag Antibody. | |
| Purification_Method: | ||
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
| Dilution: | WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200) | |
| Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. | |
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