Rabbit anti-EPHA2 (pY588/596) Antibody


Product name: | Rabbit anti-EPHA2 (pY588/596) Antibody | |
Catalog: | DL93632A |
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Synonyms : | EPHA2; ECK; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA3; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1; EPHA4; HEK8; SEK; TYRO1; Ephrin type-A receptor 4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK | |
Reactivity: | H, M, R, C, Mk | |
Applications: | WB, IF/IC | |
Size: | 50uL/100uL | |
Host: | Rabbit | |
Clonality: | Polyclonal | |
Concentration: | 1mg/mL | |
Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPHA2. The exact sequence is proprietary. | |
WB description: | Western blot analysis of EPHA2 (pY588/596) expression in A431 (A), mouse brain (B) whole cell lysates. | |
IF/ICC description: | Immunofluorescent analysis of EPHA2 (pY588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
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Purification_Method: | The antibody was purified by immunogen affinity chromatography. | |
Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. | |
Dilution: | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) | |
Gene ID(Human): | 1969; 2042; 2043 | |
Gene ID (Mouse)): | 13836; 13838 | |
Gene ID(Rat): | 29210 | |
SwissProt (Human): | P29317; P29320; P54764 | |
Storage: | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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