Rabbit anti-SMAD3 Antibody

Product name:	Rabbit anti-SMAD3 Antibody
Catalog:	DL93246A
Synonyms :	MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Reactivity:	H, M, R, D, Mk, P, S
Applications:	WB, IHC
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD3. The exact sequence is proprietary.
WB description:	Western blot analysis of SMAD3 expression in SKOV3 (A), HeLa (B), MCF7 (C) whole cell lysates.
IHC description:	Immunohistochemical analysis of SMAD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene ID(Human):	4088
Gene ID (Mouse)):	17127
Gene ID(Rat):	25631
SwissProt (Human):	P84022
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL93246A