Rabbit anti-LRP11 Antibody

Product name:	Rabbit anti-LRP11 Antibody
Catalog:	DL91823A
Synonyms :	Low-density lipoprotein receptor-related protein 11; LRP-11
Reactivity:	H
Applications:	WB, IP
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LRP11. The exact sequence is proprietary.
WB description:	Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates.
IP description:	Immunoprecipitation of LRP11 from 0.5mg Jurkat whole cell extract lysate, using 5ug of anti-LRP11 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70℃; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-LRP11 Antibody.
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/1000), IP (1/10 - 1/100)
Gene ID(Human):	84918
SwissProt (Human):	Q86VZ4
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL91823A