Rabbit anti-Cytokeratin 7 Antibody

Product name:	Rabbit anti-Cytokeratin 7 Antibody
Catalog:	DL90657A
Synonyms :	SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
Reactivity:	H
Applications:	WB, IHC, IF/IC
Size:	50uL/100uL
Host:	Rabbit
Clonality:	Polyclonal
Concentration:	1mg/mL
Immunogen:	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 7. The exact sequence is proprietary.
WB description:	Western blot analysis of Cytokeratin 7 expression in HeLa (A), A431 (B) whole cell lysates.
IHC description:
IF/ICC description:	Immunofluorescent analysis of Cytokeratin 7 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Purification_Method:	The antibody was purified by immunogen affinity chromatography.
Buffer:	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:	WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human):	3855
SwissProt (Human):	P08729
Storage:	Store at -20℃. Avoid repeated freeze / thaw cycles.

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DL90657A