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Home > Polyclonal & monoclonal

Rabbit anti-CDK1/2/3 (pT14) Antibody

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Product name: Rabbit anti-CDK1/2/3 (pT14) Antibody
Catalog:

DL90203A

Synonyms : CDK1; CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK3; CDKN3; Cyclin-dependent kinase 3; Cell division protein kinase 3
Reactivity: H, M, R, B, C, Mk
Applications: WB, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK1/2/3. The exact sequence is proprietary.
WB description: Western blot analysis of CDK1/2/3 (pT14) expression in HT29 hydroxyurea-treated (A), HeLa (B), NIH3T3 (C), A431 (D) whole cell lysates.
IF/ICC description:

Immunofluorescent analysis of CDK1/2/3 (pT14) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Gene ID(Human): 1017
Gene ID (Mouse)): 12534; 12566
Gene ID(Rat): 54237
SwissProt (Human): P06493; P24941; Q00526
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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