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Home > Polyclonal & monoclonal

Rabbit anti-CD1e Antibody

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Product name: Rabbit anti-CD1e Antibody
Catalog:

DL90176A

Synonyms : T-cell surface glycoprotein CD1e, membrane-associated; hCD1e; R2G1; CD1e
Reactivity: H, M, R
Applications: WB, IF/IC
Size: 50uL/100uL
Host: Rabbit
Clonality: Polyclonal
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD1e. The exact sequence is proprietary.
WB description: Western blot analysis of CD1e expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.
IF/ICC description:

Immunofluorescent analysis of CD1e staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ℃ in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Purification_Method: The antibody was purified by immunogen affinity chromatography.
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Gene ID(Human): 913
SwissProt (Human): P15812
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.

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